STAINING BACTERIAL SMEARS WITH FLUORESCENT ANTIBODY
نویسندگان
چکیده
منابع مشابه
Staining bacterial smears with fluorescent antibody. III. Antigenic analysis of Salmonella typhosa by means of fluorescent antibody and agglutination reactions.
Moody et al. (1956) and Thomason et al. (1956) have applied fluorescent antibody techniques to the specific detection of Malleomyces pseudomallei in dried smears. This method already has proved successful for the detection and identification of several other pathogenic bacteria. It is potentially a valuable tool for the identification of pathogenic bacteria in clinical specimens and should be o...
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The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....
متن کاملStudies on Fluorescent Antibody Staining
1. A study has been made of the non-specific fluorescent staining of splenic imprints treated with fluorescent sheep antibody globulins. 2. In tissue imprints made with the spleens of antigen-stimulated animals, no morphological distinction was evident between areas showing non-specific fluorescence and specific fluorescence. 3. Elimination of non-specific fluorescence was not achieved by any o...
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For direct observation of microaerophilic actinomycetes by fluorescent antibody, a procedure was developed in which pepsin treatment and rhodamine conjugate of normal serum were used to reduce nonspecific staining in cervicovaginal smears. Actinomyces israelii, Actinomyces naeslundii, and Arachnia propionica were observed in cervicovaginal smears from women who did use and who did not use an in...
متن کاملUse of fluorescent-antibody staining of cytocentrifuge-prepared smears in combination with cell culture for direct detection of respiratory viruses.
Over a 3-year period, 1,003 respiratory samples were collected and examined for selected respiratory viruses with cytocentrifuged prepared smears stained with fluorescently labeled antibodies (IFA) in conjunction with cell culture. IFA results were compared with results obtained by cell culture. Viruses were isolated or detected by direct means in 401 samples. Agreement between culture and IFA ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1957
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.74.4.525-532.1957